|
KMID : 0385520140270060269
|
|
Analytical Science & Technology
2014 Volume.27 No. 6 p.269 ~ p.276
|
|
|
A short study of uncertainty for post column isotope dilution method in HPLC-ICP/MS
|
|
Joo Min-gyu
Pak Yong-Nam
|
|
|
Abstract
|
|
|
A short study for the uncertainty of post column isotope dilution method has been performed for the analysis of Selenomethionine in HPLC-ICP/MS. Major error sources studied were concentration and the flow rate of Se isotope solution, atomic weights of Se in spike and sample, and isotope ratio measured for the spiked sample. Uncertainties were obtained for each factor and the contribution for the total concentration uncertainty was 54.4% and 0.61%, 0.0072% and 0.018%, and 45.0%, respectively. The biggest contribution factor was concentration of the spike solution and the second was the isotopic ratio measured for the spiked sample solution. The mass flow rate of spike and atomic weights did not show much contribution. The calculated total uncertainty was 1.46 ng·g?1 for the standard SeMet (126.30 ng·g?1
). The experimental result was 127.09±1.46 ng·g?1 and the relative uncertainty was 1.20%.
|
|
|
KEYWORD
|
|
|
species unspecific isotope dilution, uncertainty, error calculation, HPLC-ICP/MS,
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|